KOBE UNIVERSITY, FACULTY OF AGRICULTURE

Laboratory of Reproductive Biology and Biotechnology

Last Update July 27, 2003

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　研究室ゼミのご案内　（2003年度）

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　4/12(Sat.)　新人歓迎会（♪花見＆梅山豚BBQパーティー♪）

　4/15(Tue.)　卒業研究の紹介（神戸大学農学部　B303　15：30〜）

• 植戸　登「ブタ成熟卵母細胞へ顕微注入した体細胞核の変化」
• 木村　隼人「マウス初期胚で発現する遺伝子群（DDクローン）のIRES発現ベクターを利用した機能解析」
• 小野　光樹子「ブタ卵胞卵の成熟率に及ぼす卵巣の保存の影響；保存液」

　4/22(Tue.)　第４回CDB合同セミナー/COE学術講演会　(神戸大学遺伝子実験センター／５F研修室　15：00〜)
　　''Frog Egg and Mammalian Egg''

• ''Fertilization Signaling : Lessons from Xenopus Eggs''
  Ken-ichi Sato(Faculty of science, Kobe University)
• ''Signal Transduction during Pig Oocyte Maturation and Activation''
  Qing-Yuan Sun (Institute of Zoology, Chinese Academy of Sciences)
• ''Nucleus Transfer in Mammals : Noninvasive Approaches for the Preparation of Cytoplasts''
  Josef Fulka,Jr* and Takashi(TAKU)Nagai** (* Institute of Animal Production, Czech Republic,**National Institute of Agrobiological Sciences, Japan)

　4/29(Tue.)　ゼミはお休みです♪

　 5/6(Tue.)　文献紹介（神戸大学農学部　B303　15：30〜）

• McCoard SA, Wise TH, Ford JJ.　
  "Germ cell development in Meishan and White Composite gilts."
  Anim Reprod Sci. 2003 May 15;77(1-2):85-105. ／室賀　真衣
• Van den Broecke R, Liu J, Handyside A, Van der Elst JC, Krausz T, Dhont M, Winston RM, Hovatta O.
  "Follicular growth in fresh and cryopreserved human ovarian cortical grafts transplanted to immunodeficient mice."
  Eur J Obstet Gynecol Reprod Biol. 2001 Aug;97(2):193-201. ／石井　恭輔

　 5/13(Tue.)　文献紹介（神戸大学農学部　B303 15:30〜）

• Gall L, Ruffini S, Le Bourhis D, Boulesteix C.
  "Cdc25C expression in meiotically competent and incompetent goat oocytes."
  Mol Reprod Dev. 2002 May;62(1):4-12. ／石川　友香
• Elhassan YM, Wu G, Leanez AC, Tasca RJ, Watson AJ, Westhusin ME.
  "Amino acid concentrations in fluids from the bovine oviduct and uterus and in KSOM-based culture media."
  Theriogenology. 2001 Jun 1;55(9):1907-18. ／佐井　敦子

　5/20(Tue.)　文献紹介（神戸大学農学部　B303　15：30〜）

• O'Brien MJ, Pendola JK, Eppig JJ.
  "A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence."
  Biol Reprod. 2003 May;68(5):1682-6. ／M.Moniruzzaman
• Ou YY, Mack GJ, Zhang M, Rattner JB.
  "CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation."
  J Cell Sci. 2002 May 1;115(Pt 9):1825-35. ／植戸　登

　5/27（Tue.) 　第５回CDB合同セミナー（英語）　15：30〜　
　(理化学研究所発生・再生科学総合研究センター C棟４階セミナー室)

• "Male Germ Cell Transplantation in Mouse"
  Hiroshi Ohta (Laboratory for Genomic Reprogramming, RIKEN Kobe Institute, Center for Developmental Biology)
• "Chromosome cohesion and separation during mammalian meiosis: Meiosis-specific cohesin Rec8"
  Jabak Lee (Department of Life Science, Graduate School of Science and Technology, Kobe University)

　6/3（Tue.) 　文献紹介（神戸大学農学部　B303　15：30〜）

• de Lamirande E, Gagnon C.
  "The extracellular signal-regulated kinase (ERK) pathway is involved in human sperm function and modulated by the superoxide anion."
  Mol Hum Reprod. 2002 Feb;8(2):124-35.／黒田　顕
• Miller DJ, Eckert JJ, Lazzari G, Duranthon-Richoux V, Sreenan J, Morris D, Galli C, Renard JP, Fleming TP.
  "Tight Junction Messenger RNA Expression Levels in Bovine Embryos are Dependent upon the Ability to Compact and In Vitro Culture Methods."
  Biol Reprod. 2003 Apr;68(4):1394-402.／木村　隼人

　6/10（Tue.)　文献紹介（神戸大学農学部　B303　15：30〜）

• de Rooij J, Zwartkruis FJ, Verheijen MH, Cool RH, Nijman SM, Wittinghofer A, Bos JL.
  "Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP."
  Nature. 1998 Dec 3;396(6710):474-7. ／天野　玲子
• Lee JW, Tian XC, Yang X.
  "Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection."
  Biol Reprod. 2003 Apr;68(4):1341-7. Epub 2002 Oct 30. ／久保　麻世

　6/17（Tue.)　文献紹介（神戸大学農学部　B303 15:30〜）

• Nagy A, Gocza E, Diaz EM, Prideaux VR, Ivanyi E, Markkula M, Rossant J.
  "Embryonic stem cells alone are able to support fetal development in the mouse."
  Development. 1990 Nov;110(3):815-21. ／藤原　祥高
• Fan HY, Tong C, Lian L, Li SW, Gao WX, Cheng Y, Chen DY, Schatten H, Sun QY.
  "Characterization of Ribosomal S6 Protein Kinase p90rsk During Meiotic Maturation and Fertilization in Pig Oocytes: Mitogen-Activated Protein Kinase-Associated Activation and Localization."
  Biol Reprod. 2003 Mar;68(3):968-77. ／萩野　輝

　6/24（Tue.)　第6回CDB合同セミナー　15：00〜　(神戸大学農学部／B303)

• "ICSI in the pig (Where is the problem?)
  Radomir KREN
• "The study of bovine oocyte growth using in vitro culture and xenografting"
  Shoichiro Senbon

　7/1 (Tue.)　文献紹介（神戸大学農学部　B303　15：30〜）

• Yuzi Hirao, Ken-ichi Aizawa, Naoki Takenouchi and Takashi Nagai
  "Effects of oxygen Tension on Survival and Growth In Vitro of Bovine Oocytes from Early Antral Follicles"
  J.Mamm. Ova Res. Vol.19, 12-20, 2002／松本　伊代
• Pommer AC, Rutllant J, Meyers SA.
  "Phosphorylation of Protein Tyrosine Residues in Fresh and Cryopreserved Stallion Spermatozoa under Capacitating Conditions."
  Biol Reprod. 2003 Apr;68(4):1208-14. ／田渕　智士

　7/8 (Tue.)　修士論文中間報告＆文献紹介（神戸大学農学部　B303 15:30〜）

• M.Moniruzzaman「Growth of small oocytes in primordial follicles from adult and newborn pigs in SCID mice」
• Wei Y, Mizzen CA, Cook RG, Gorovsky MA, Allis CD.
  Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena.
  Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7480-4. ／松下　昌樹

　7/15 (Tue.)　修士論文中間報告　（神戸大学農学部　B303 15:30〜）

• 石川　友香「ブタ卵母細胞の発育過程における減数分裂再開能力の獲得」
• 石井　恭輔「SCIDマウスへの異種移植による凍結保存ウシ二次卵胞の発達」

　7/22 (Tue.)　修士論文中間報告　（神戸大学農学部　B303 15:30〜）

• 室賀　真衣「ブタ生殖腺における減数分裂型コヒーシンRec8の発現」
• 佐井　敦子「分子鎖アミノ酸のブタ単為発生２倍体の初期発生に及ぼす影響とその作用機構」

　9/22 (Tue.)　第7回CDB合同セミナー(神戸大学遺伝子実験センター／５F研修室　15：00〜)

• "The role of germinal vesicle materials in maturation and fertilization of pig oocytes"
  Sugako Ogushi (Department of Life Science, Graduate School of Science and Technology,Kobe University)
• "Tr-kit promotes the formation of a multimolecular complex formed by Fyn, PLCg1 and Sam68"
  Claudio Sette (Department of Public Health and Cell Biology, University of Rome "Tor Vergata", ITALY)

　 10/7 (Tue.) 　文献紹介（神戸大学農学部　B303 15:30〜）

• Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, Okawa K, Iwamatsu A, Okigaki T, Takahashi T, Inagaki M.
  "Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation."
  J Biol Chem. 1999 Sep 3;274(36):25543-9. ／松下　昌樹
• Enserink JM, Christensen AE, de Rooij J, van Triest M, Schwede F, Genieser HG, Doskeland SO, Blank JL, Bos JL.
  "A novel Epac-specific cAMP analogue demonstrates independent regulation of Rap1 and ERK."
  Nat Cell Biol. 2002 Nov;4(11):901-6. ／天野　玲子

　 10/14 (Tue.)　文献紹介（神戸大学農学部　B303 15:30〜）

• A.Shimada, K.Kikuchi, J.Noguchi, K.Akama, M.Nakano, H.kaneko.
  "protamin dissocitation before decondensation of sperm muclei during in vitro fertilization of pig oocytes"
  J. Reprod. Fert. 2000 120:247-256. ／久保　麻世
• Brener E, Rubinstein S, Cohen G, Shternall K, Rivlin J, Breitbart H.
  Remodeling of the actin cytoskeleton during mammalian sperm capacitation and acrosome reaction.
  Biol Reprod. 2003 Mar;68(3):837-45. ／黒田　顕

　 10/21(Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Kagii H, Naito K, Sugiura K, Iwamori N, Ohashi S, Goto S, Yamanouchi K, Tojo H.
  "Requirement of Mitogen-activated Protein Kinase Activation for the Meiotic resumption of Porcine Oocytes"
  J. Reprod. Dev. 2000 Aug;46(4):249-256／松本　伊代
• Fukami K, Yoshida M, Inoue T, Kurokawa M, Fissore RA, Yoshida N, Mikoshiba K, Takenawa T.
  "Phospholipase Cdelta4 is required for Ca2+ mobilization essential for acrosome reaction in sperm."
  J Cell Biol. 2003 Apr 14;161(1):79-88. ／田渕　智士

　 10/28 (Tue.)　第８回CDB合同セミナー(理化学研究所発生・再生科学総合研究センター C棟6階セミナー室 15：30〜)

• "Growth of Oocytes in Pig Primordial Follicles Xenotransplanted into SCID Mice"
  Mohammad Moniruzzaman(Graduate School of Science and Technology, Kobe University)
• "Microfluidics: A New Tool for CellBiology, Transgenics, and Assisted Reproductive Technologies"
  Gary D. Smith, Ph.D.( Associate Professor, Departments of Ob/GYN, Urology, and Molecular and Integrated Physiology, University of Michigan Medical School)

　 11/3 (Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Sugiura K, Naito K, Iwamori N, Kagii H, Goto S, Ohashi S, Naruoka H, Yada E, Yamanouchi K, Tojo H.
  Activation of ribosomal S6 kinase (RSK) during porcine oocyte maturation.
  Zygote. 2002 Feb;10(1):31-6.／萩野　輝
• Everett CA, West JD.
  The influence of ploidy on the distribution of cells in chimaeric mouse blastocysts.
  Zygote. 1996 Feb;4(1):59-66.／藤原　祥高

　 11/11 (Tue.)　卒業論文中間報告（神戸大学農学部 B303 15:30〜）

• 黒田　顕　「ブタ精子のキャパシテーション過程におけるタンパク質のセリン／スレオニンリン酸化」
• 松下　昌樹「ブタ卵母細胞成熟過程で起こる染色体凝縮とヒストンのリン酸化」
• 天野　玲子「ブタ精子における新規cAMPシグナリング経路の検索」
• 久保　麻世「精子の界面活性剤処理が雄性前核形成率に及ぼす影響」

　11/18(Tue.) 　卒業論文中間報告　(神戸大学農学部 B303 15:30〜)

• 萩野　輝　「ブタ卵母細胞の成熟過程におけるMAPKおよびリボソームS6キナーゼの変化」
• 藤原　祥高「マウス胚盤胞形成過程における2倍体ならびに4倍体細胞の動態」
• 松本　伊代「オカダ酸による発育途上ブタ卵母細胞の減数分裂再開機構」
• 田渕　智士「ブタ精子における32kDaタンパク質チロシンリン酸化と先体反応との関係」

　11/25(Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Meraldi P, Lukas J, Fry AM, Bartek J, Nigg EA.
  Centrosome duplication in mammalian somatic cells requires E2F and Cdk2-cyclin A.
  Nat Cell Biol. 1999 Jun;1(2):88-93. ／植戸　登
• Rangarajan S, Enserink JM, Kuiperij HB, de Rooij J, Price LS, Schwede F, Bos JL.
  Cyclic AMP induces integrin-mediated cell adhesion through Epac and Rap1 upon stimulation of the beta 2-adrenergic receptor.
  J Cell Biol. 2003 Feb 17;160(4):487-93. Epub 2003 Feb 10. ／天野　玲子
• Phosphorylation of serine 10 in histone H3, what for?
  J Cell Sci. 2003 Sep 15;116(Pt 18):3677-85. ／松下　昌樹

　12/2(Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Ahmadi A, Ng SC.
  Developmental capacity of damaged spermatozoa.
  Hum Reprod. 1999 Sep;14(9):2279-85.／久保　麻世
• Jaiswal BS, Conti M.
  Calcium regulation of the soluble adenylyl cyclase expressed in mammalian spermatozoa.
  Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10676-81. Epub 2003 Sep 04. ／黒田　顕
• Yoshioka K, Suzuki C, Tanaka A, Anas IM, Iwamura S.
  Birth of piglets derived from porcine zygotes cultured in a chemically defined medium.
  Biol Reprod. 2002 Jan;66(1):112-9. ／木村　隼人

　12/9(Tue.)　第9回CDB合同セミナー/COE学術講演会　(神戸大学遺伝子実験センター／５F研修室　15：30〜)

• ''哺乳類精子ファクターとphospholipase"
  尾田正二（東京大学大学院新領域創成科学研究科）

　12/16(Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Ye J, Flint AP, Luck MR, Campbell KH.
  Independent activation of MAP kinase and MPF during the initiation of meiotic maturation in pig oocytes.
  Reproduction. 2003 May;125(5):645-56. ／松本伊予
• Kirkman-Brown JC, Barratt CL, Publicover SJ.
  Nifedipine reveals the existence of two discrete components of the progesterone-induced [Ca2+]i transient in human spermatozoa.
  Dev Biol. 2003 Jul 1;259(1):71-82. ／田淵智士

　12/22 (Mon.)　第10回CDB合同セミナー(理化学研究所発生・再生科学総合研究センター C棟6階セミナー室 15：30〜)＋忘年会

• "Development of bovine secondary follicles after xenotransplantation and vitrification"
  Kyosuke Ishii(Graduate School of Science and Technology, Kobe University)
• "Effect of branched-chain amino acids on porcine embryo development"
  Atsuko Sai(Graduate School of Science and Technology, Kobe University)
• "Acquisition of meiotic competence during growth of pig oocytes"
  Yuka Ishikawa(Graduate School of Science and Technology, Kobe University)
• "Expression of meiosis-specific cohesin subunit Rec8 in pig gonads"
  Mai Muroga(Graduate School of Science and Technology, Kobe University)

☆学会，研究会，セミナーなど今年も１年間お疲れさまでした☆

※1/27のCDB合同セミナーはありません，次回は２月からです。1/26〜28に行われる国際セミナーに参加しましょう。

　　明けましておめでとうございます。今年も実験などがんばりましょう！

　1/13(Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Tang PC, Ritchie WA, Wilmut I, West JD.
  The effects of cell size and ploidy on cell allocation in mouse chimaeric blastocysts.
  Zygote. 2000 Feb;8(1):33-43. ／藤原祥高
• Bandyopadhyay A, Bandyopadhyay J, Chung J, Choi HS, Kwon HB.
  Inhibition of S6 kinase by rapamycin blocks maturation of Rana dybowskii oocytes.
  Gen Comp Endocrinol. 1999 Feb;113(2):230-9. ／萩野輝

　1/20(Tue.)　文献紹介（神戸大学農学部 B303 15:30〜）

• Wianny F, Zernicka-Goetz M.
  Specific interference with gene function by double-stranded RNA in early mouse development.
  Nat Cell Biol. 2000 Feb; 2(2): 70-5.／小野光樹子
• Sheth B, Fesenko I, Collins JE, Moran B, Wild AE, Anderson JM, Fleming TP.
  Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha+ isoform.
  Development. 1997 May; 124(10): 2027-37.／木村隼人
• Gao S, Gasparrini B, McGarry M, Ferrier T, Fletcher J, Harkness L, De Sousa P, Wilmut I.
  Germinal vesicle material is essential for nucleus remodeling after nuclear transfer.
  Biol Reprod. 2002 Sep; 67(3): 928-34. ／植戸登

　2/20(Fri.)　修論発表会

• 佐井敦子「分子鎖アミノ酸がブタ初期発生に及ぼす影響」
• 室賀真衣「ブタ生殖腺における減数分裂型コヒーシンサブユニットRec8の発現」
• 石井恭輔「ウシ二次卵胞の異種移植による発達とガラス化保存」
• 石川友香「ブタ卵母細胞の発育過程における減数分裂再開能力の獲得」
• M. Moniruzzaman「Growth of oocyte in porcine primordial follicles xenotransplanted into SCID mice」

　3/17(Wed.)　第11回CDB合同セミナー(理化学研究所発生・再生科学総合研究センター C棟6階セミナー室 15：30〜)

　　　"Frog Egg & Mammalian Egg, 2nd"

• "Initiation and persistence of fertilization-associated Ca2+ oscillations in mouse eggs: Roles of the sperm factor and IP3R-1"
  Dr. Rafael A. Fissore (Department of Veterinary and Animal Sciences, University of Massachusetts)
• "Xenopus oocyte expression system - old dog, new tricks"
  Dr. Alexander A. Tokmakov (Protein research group, Genomic Sciences Center, RIKEN Yokohama Institute)
• "A novel method for long-term preservation of mouse spermatozoa without freezing"
  Dr. Van Thuan Nguyen (Laboratory of Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe)

　3/24(Wed)　卒業式

　　おめでとうございます，実験，卒論，修論お疲れさまでした。

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