KOBEUNIVERSITY, FACULTY OF AGRICULTURE

 

Laboratory of Reproductive Biology and Biotechnology

 

Last Update Dec 15, 2009

 


 研究室ゼミのご案内 (2009年度)


生殖生物・発生工学研究室ゼミの日程  場所:神戸大学農学部(B201, B202 

Apr 14 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Nishimura Y, Endo T, Kano K, Naito K. Porcine Aurora A accelerates Cyclin B and Mos synthesis and promotes meiotic resumption of porcine oocytes. Anim Reprod Sci. 2009, in press./ Ilse S. Cayo (D2)

Hirao Y, Shimizu M, Iga K, Takenouchi N. Growth of bovine oocyte-granulosa cell complexes cultured individually in microdrops of various sizes. J Reprod Dev. 2009 Feb;55(1):88-93./ 竹鶴 裕亮 (D2)

 

Apr 21 (Tue) 研究概要説明 (神戸大学農学部 1510〜)

・三宅 正史 (P)

・佐東 春香 (M1) 卒業研究の内容紹介

 

Apr 24 (Fri) 研究概要説明 (神戸大学農学部 1040〜)

・原山 洋 (AP) 哺乳類精子での部位特異的なcAMPシグナリング経路(2008年度に精子組がやったこと?)

・宮野 隆 (P) 

 

May 1 (Fri) 実験報告(神戸大学農学部 1040〜)

・野田 大地 (M2)

・平嶋 友美子 (M2)

・山上 由希子 (M2)

 

May 12 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Rodova M, Nguyen AN, Blanco G. The transcription factor CREMtau and cAMP regulate promoter activity of the Na,K-ATPase alpha4 isofor m. Mol Reprod Dev. 2006 Nov;73(11):1435-47./野田 大地 (M2)

Orisaka M, Jiang JY, Orisaka S, Kotsuji F, Tsang BK. Growth Differentiation Factor 9 Promotes Rat Preantral Follicle Growth by Up-regulating Follicular Androgen Biosynthesis. Endocrinology. 2009 Feb 12. [Epub ahead of print](in press)/ Sumith Magamage (D2)

Neganova I, Al-Qassab H, Heffron H, Selman C, Choudhury AI, Lingard SJ, Diakonov I, Patterson M, Ghatei M, Bloom SR, Franks S, Huhtaniemi I, Hardy K, Withers DJ. Role of central nervous system and ovarian insulin receptor substrate 2 signaling in female reproductive function in the mouse. Biol Reprod. 2007 Jun;76(6):1045-53./ 平嶋 友美子 (M2)

 

May 19 (Tue) 文献紹介 (神戸大学農学部 1510〜)

・新型インフルエンザ流行のため,延期

 

May 26 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Bao S, Ushijima H, Hirose A, Aono F, Ono Y, Kono T. Development of bovine oocytes reconstructed with a nucleus from growing stage oocytes after fertilization in vitro. Theriogenology. 2003 Mar;59(5-6):1231-9./ 京極 博久 (M1)

Wang WH, Abeydeera LR, Prather RS, Day BN. Polymerization of nonfilamentous actin into microfilaments is an important process for porcine oocyte maturation and early embryo development. Biol Reprod. 2000 May;62(5):1177-83./ 高橋 典子 (M1)

Morgan DJ, Weisenhaus M, Shum S, Su T, Zheng R, Zhang C, Shokat KM, Hille B, Babcock DF, McKnight GS. Tissue-specific PKA inhibition using a chemical genetic approach and its application to studies on sperm capacitation. Proc Natl Acad Sci USA. 2008 Dec 30;105(52):20740-5./ 綿井 佑輔 (M1)

 

May 29 (Fri) 実験報告(神戸大学農学部 1040〜)

・竹鶴 裕亮 (D2)

・包 栄梅 (D2)

 

June 2 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Korhonen K, Kananen K, Ketoja E, Matomäki J, Halmekytö M, Peippo J. Effects of serum-free in vitro maturation of bovine oocytes on subsequent embryo development and cell allocation in two developmental stages of day 7 blastocysts. Reprod Domest Anim. 2008 Dec 12. [Epub ahead of print]/ 佐東 春香 (M1)

Lucidi P, Bernabò N, Turriani M, Barboni B, Mattioli M. Cumulus cells steroidogenesis is influenced by the degree of oocyte maturation. Reprod Biol Endocrinol. 2003 May 28;1:45./ 山上 由希子 (M2)

 

June 5 (Fri) 実験報告(神戸大学農学部 1040〜)

Brahamajosyula Manjula (D3)

・京極 博久 (M1)

・綿井 佑輔 (M1)

 

June 9 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Xu M, Banc A, Woodruff TK, Shea LD. Secondary follicle growth and oocyte maturation by culture in alginate hydrogel following cryopreservation of the ovary or individual follicles. Biotechnol Bioeng. 2009 Jun 1;103(2):378-86./ 包 栄梅 (D2)

Wongsrikeao P, Otoi T, Yamasaki H, Agung B, Taniguchi M, Naoi H, Shimizu R, Nagai T. Effects of single and double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos. Theriogenology. 2006 Jul 15;66(2):366-72./ 渋谷 海大 B4

 

June 16 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Wang Y, Li M, Stadler S, Correll S, Li P, Wang D, Hayama R, Leonelli L, Han H, Grigoryev SA, Allis CD, Coonrod SA. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J Cell Biol. 2009 Jan 26;184(2):205-13./ Brahamajosyula Manjula (D3

Da Silva-Buttkus P, Jayasooriya GS, Mora JM, Mobberley M, Ryder TA, Baithun M, Stark J, Franks S, Hardy K.. Effect of cell shape and packing density on granulosa cell proliferation and formation of multiple layers during early follicle development in the ovary. J Cell Sci. 2008 Dec 1;121(Pt 23):3890-900./ Sumith Magamage D2

 

June 23 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Norris RP, Freudzon M, Mehlmann LM, Cowan AE, Simon AM, Paul DL, Lampe PD, Jaffe LA. Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption. Development. 2008 Oct;135(19):3229-38./ Ilse S. Cayo (D2

John GB, Shidler MJ, Besmer P, Castrillon DH. Kit signaling via PI3K promotes ovarian follicle maturation but is dispensable for primordial follicle activation. Dev Biol. 2009 May 15. [Epub ahead of print]/ 平嶋 友美子 (M2)

Zarelli VE, Ruete MC, Roggero CM, Mayorga LS, Tomes CN. PTP1B dephosphorylates N-ethylmaleimide-sensitive factor and elicits SNARE complex disassembly during human sperm exocytosis. J Biol Chem. 2009 Apr 17;284(16):10491-503./ 松下 夕希 M1

 

June 26 (Fri) 実験報告(神戸大学農学部 1040〜)

Sumith Magamage D2

・高橋 典子 (M1)

・佐東 春香 (M1)

 

June 30 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Xu M, West-Farrell ER, Stouffer RL, Shea LD, Woodruff TK, Zelinski MB. Encapsulated three-dimensional culture supports development of nonhuman primate secondary follicles. Biol Reprod. 2009 Sep;81(3):587-94./ 竹鶴 裕亮 (D2)

Obata Y, Kono T. Maternal primary imprinting is established at a specific time for each gene throughout oocyte growth. J Biol Chem. 2002 Feb 15;277(7):5285-9./ 京極 博久 (M1)

Kim JS, Cho YS, Song BS, Wee G, Park JS, Choo YK, Yu K, Lee KK, Han YM, Koo DB. Exogenous dibutyryl cAMP affects meiotic maturation via protein kinase A activation; it stimulates further embryonic development including blastocyst quality in pigs.Theriogenology. 2008 Feb;69(3):290-301./ 山上 由希子 (M2)

 

July 3 (Fri) 実験報告(神戸大学農学部 1040〜)

・松下 夕希 M1

・坂下 弘道 B4

・渋谷 海大 B4

 

July 14 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Esposito G, Vitale AM, Leijten FP, Strik AM, Koonen-Reemst AM, Yurttas P, Robben TJ, Coonrod S, Gossen JA. Peptidylarginine deiminase (PAD) 6 is essential for oocyte cytoskeletal sheet formation and female fertility. Mol Cell Endocrinol. 2007 Jul 15;273(1-2):25-31./ Brahamajosyula Manjula (D3

Jaiswal BS, Conti M. Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase. J Biol Chem. 2001 Aug 24;276(34):31698-708./ 綿井 佑輔 (M1)

Kurokawa M, Sato K, Wu H, He C, Malcuit C, Black SJ, Fukami K, Fissore RA. Functional, biochemical, and chromatographic characterization of the complete [Ca2+]i oscillation-inducing activity of porcine sperm. Dev Biol. 2005 Sep 15;285(2):376-92./ 坂下 弘道 B4

 

July 17 (Fri) 実験報告(神戸大学農学部 1040〜)

M2修士論文中間発表会

 

July 21 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Vouk K, Hudler P, Strmsnik L, Fink M, Majdic G, Zorn B, Lalli E, Sassone-Corsi P, Debeljak N, Komel R, Rozman D. Combinations of genetic changes in the human cAMP-responsive element modulator gene: a clue towards understanding some forms of male infertility? Mol Hum Reprod. 2005 Aug;11(8):567-74./ 野田 大地 M2

Yoshioka K, Oh KB, Saito M, Nemoto Y, Matsuoka H. Evaluation of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose, a new fluorescent derivative of glucose, for viability assessment of yeast Candida albicans. Appl Microbiol Biotechnol. 1996 Nov;46(4):400-4./ 渋谷 海大 B4

 

July 24 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Oikonomopoulou I, Patel H, Watson PF, Chantler PD. Relocation of myosin and actin, kinesin and tubulin in the acrosome reaction of bovine spermatozoa. Reprod Fertil Dev. 2009;21(2):364-77./ 松下 夕希 M1

Yamagata K, Suetsugu R, Wakayama T. Long-term, six-dimensional live-cell imaging for the mouse preimplantation embryo that does not affect full-term development. J Reprod Dev. 2009 Jun;55(3):343-50./ 高橋 典子 (M1)

Bomar J, Moreira P, Balise JJ, Collas P. Differential regulation of maternal and paternal chromosome condensation in mitotic zygotes. J Cell Sci. 2002 Jul 15;115(Pt 14):2931-40./ 佐東 春香 (M1)

 Kurokawa M, Yoon SY, Alfandari D, Fukami K, Sato K, Fissore RA. Proteolytic processing of phospholipase Czeta and [Ca2+]i oscillations during mammalian fertilization. Dev Biol. 2007 Dec 1;312(1):407-18./ 坂下 弘道 B4

 

Oct 6 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Rossetto R, Lima-Verde IB, Matos MH, Saraiva MV, Martins FS, Faustino LR, Araújo VR, Silva CM, Name KP, SN SN, Campello CC, Figueiredo JR, Blume H. Interaction between ascorbic acid and follicle-stimulating hormone maintains follicular viability after long-term in vitro culture of caprine preantral follicles. Domest Anim Endocrinol. 2009 Aug;37(2):112-23./ 竹鶴 裕亮 (D2)

He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation. Stem Cells. 2008 Jan;26(1):266-78./ 野田 大地 M2

 

Oct 13 (Tue) 文献紹介 (神戸大学農学部 1510〜)

 Huang Z, Vijayaraghavan S. Increased phosphorylation of a distinct subcellular pool of protein phosphatase, PP1gamma2, during epididymal sperm maturation. Biol Reprod. 2004 Feb;70(2):439-47./ 綿井 佑輔 (M1)

 

Oct 20 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Liu H, Luo LL, Qian YS, Fu YC, Sui XX, Geng YJ, Huang DN, Gao ST, Zhang RL. FOXO3a is involved in the apoptosis of naked oocytes and oocytes of primordial follicles from neonatal rat ovaries.Biochem Biophys Res Commun. 2009 Apr 17;381(4):722-7/ 平嶋 友美子 (M2)

Bjerregaard B, Wrenzycki C, Strejcek F, Laurincik J, Holm P, Ochs RL, Rosenkranz C, Callesen H, Rath D, Niemann H, Maddox-Hyttel P. Expression of nucleolar-related proteins in porcine preimplantation embryos produced in vivo and in vitro. Biol Reprod. 2004 Apr;70(4):867-76./ 山上 由希子 (M2)

Oct 20 (Tue) 実験報告(神戸大学農学部 15:10〜)

 Sumith Magamage (D2)

 

Nov 10 (Tue) 実験報告(神戸大学農学部 15:10〜)

・包 栄梅 (D2)

・竹鶴 裕亮 (D2)

Ilse S. Cayo (D2)

 

Nov 17 (Tue) 実験報告(神戸大学農学部 15:10〜)

・渋谷 海大 B4

 

Nov 24 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Qureshi AI, Nussey SS, Bano G, Musonda P, Whitehead SA, Mason HD. Testosterone selectively increases primary follicles in ovarian cortex grafted onto embryonic chick membranes: relevance to polycystic ovaries. Reproduction. 2008 Aug;136(2):187-94./ Sumith Magamage (D2)

Wang S, Hu J, Guo X, Liu JX, Gao S. ADP-ribosylation factor 1 regulates asymmetric cell division in female meiosis in the mouse. Biol Reprod. 2009 Mar;80(3):555-62./ 高橋 典子 (M1)

Nov 24 (Tue) 実験報告(神戸大学農学部 15:10〜)

Brahamajosyula Manjula (D3

 

Dec 1 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Pons-Rejraji H, Bailey JL, Leclerc P. Modulation of bovine sperm signalling pathways: correlation between intracellular parameters and sperm capacitation and acrosome exocytosis. Reprod Fertil Dev. 2009;21(4):511-24./ 松下 夕希 M1

Schmitt J, Benavente R, Hodzic D, Höög C, Stewart CL, Alsheimer M. Transmembrane protein Sun2 is involved in tethering mammalian meiotic telomeres to the nuclear envelope. Proc Natl Acad Sci U S A. 2007 May 1;104(18):7426-31./  佐東 春香 (M1)

 

Dec 15 (Tue) 実験報告(神戸大学農学部 1510〜)

・坂下 弘道 B4

10分間の英語発表の方法について/宮野 隆 (P)      

 

Dec 22 (Tue) 文献紹介 (修士論文英語発表会 1510〜) Chaired by Mr. Taketsuru

Taichi Noda (野田 大地) M2

Yumiko Hirashima (平嶋 友美子) M2

Yukiko Yamagami (山上 由希子) (M2)

 

Jan 12 (Tue) 文献紹介 (神戸大学農学部 1510〜)

Brahamajosyula Manjula (D3

Ilse S. Cayo (D2)

・渋谷 海大 B4

 

Jan 19 (Tue) 実験報告(神戸大学農学部 15:10〜)

・高橋 典子 (M1)

・綿井 佑輔 (M1) 

 

Jan 26 (Tue) 実験報告(神戸大学農学部 15:10〜)

・松下 夕希 M1

・佐東 春香 (M1)