生体膜機能研究分野

Jiaying Wen, Kazuhito Sai, Suzuka Masutani, Yuki Aoshima, Kotaro Yumi, Tomoki Kyo, Junya Hasegawa, Toshiki Itoh, Ken Mizuta, Tomonori Nakamura, Yukihiro Yabuta, Mitinori Saitou, Satoshi Toda, Yasuyuki Fujita
Filamin-ETV4/5 acts as mechanosensor-mechanotransducer axis that drives cell competition-mediated elimination of transformed cells.
Nat. Commun., online ahead of print. (2026)

Ishida H, Fukuda N, Shimada M, Yamamoto K, Gaponova L, Higuchi R, Kolosiuk A, Hoshina R, Arikawa M, Fukuda Y, Islam MDS, Harumoto T, Wan Y, Itoh T, Inai Y, Takeishi A, Aonuma H, Kikuchi T, Nishigori S, Maruyama T, Ikeda K, Iriko H, Suzaki T.
Artifact-free preparation of biological samples for SEM by optimized water freeze-drying.
Sci. Rep., 16(1): 717. (2025)

Takano K, Tsujita K, Suganami A, Nakamura T, Kanno E, Tamura Y, Itoh T, Endo T.
DA-Raf synergistically binds to the plasma membrane and Ras to suppress ERK signaling.
Life Sci. Alliance, 8(12): e202503300. (2025)

Wan Y, Nemoto YL, Oikawa T, Takano K, Fujiwara TK, Tsujita K, Itoh T.
Mechanical control of osteoclast fusion by membrane-cortex attachment and BAR proteins.
J. Cell Biol., 224(7): e202411024 (2025)

Kou L, Wan Y, Nemoto YL, Tsujita K, Itoh T.
Role of membrane proximal actin regulators in SARS-CoV-2 spike-induced cell-cell fusion.
Biochem. Biophys. Res. Commun., 766:151846. (2025)

Itoh T, Tsujita K.
Exploring membrane mechanics: The role of membrane-cortex attachment in cell dynamics.
Curr. Opin. Cell Biol., 81:102173. (2023)

Yuge S, Nishiyama K, Arima Y, Hanada Y, Oguri-Nakamura E, Hanada S, Ishii T, Wakayama Y, Tsujita K, Yokokawa R, Miura T, Itoh T, Tsujita K, Mochizuki N, Fukuhara S.
Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins.
Nat. Commun., 13(1):2594. (2022)

Kanemaru K, Shimozawa M, Kitamata M, Furuishi R, Kayano H, Sukawa Y, Chiba Y, Fukuyama T, Tsujita K, Itoh T, Hasegawa J, Nakanishi H, Sasaki J, Sasaki T, Nakamura Y, Fukami K.
Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics.
Nat. Commun., 13(1):2347. (2022)

Tsujita K, Satow R, Asada S, Nakamura Y, Arnes L, Sako K, Fujita Y, Fukami K, Itoh T.
Homeostatic membrane tension constrains cancer cell dissemination by counteracting BAR protein assembly.
Nat. Commun., 12(1):5930. (2021)

Kamasaki T, Miyazaki Y, Ishikawa S, Hoshiba K, Kuromiya K, Tanimura N, Mori Y, Tsutsumi M, Nemoto T, Uehara R, Suetsugu S, Itoh T, Fujita Y.
FBP17-mediated finger-like membrane protrusions in cell competition between normal and RasV12-transformed cells.
iScience, 24, 102994. (2021)

Motegi T, Takiguchi K, Tanaka-Takiguchi Y, Itoh T, Tero R.
Physical Properties and Reactivity of Microdomains in Phosphatidylinositol-Containing Supported Lipid Bilayer.
Membranes (Basel), 11, 1-12. (2021)

Yan L, Tsujita K, Fujita Y, Itoh T.
PTEN is required for the migration and invasion of Ras‐transformed MDCK cells.
FEBS Lett., 595, 1303-1312. (2021)

Jebri I, Tsujita K, Fujita Y, Itoh T.
Non-cell-autonomous migration of RasV12-transformed cells towards the basal side of surrounding normal cells.
Biochem. Biophys. Res. Commun., 543, 15-22. (2021)

Hasegawa J, Jebri I, Yamamoto H, Tsujita K, Tokuda E, Shibata H, Maki M, Itoh T.
SH3YL1 cooperates with ESCRT-I in the sorting and degradation of the EGF receptor.
J. Cell Sci., 132(19):jcs229179 (2019)

Masuda T, Baba K, Nomura T, Tsujita K, Murayama T, Itoh T, Takatani-Nakase T, Sokabe M, Inagaki N, Futaki S.
An influenza-derived membrane tension-modulating peptide regulates cell movement and morphology via actin remodeling.
Commun Biol., 2: 243 (2019)

Sakaguchi K, Shirai Y, Itoh T, Mizuno M.
Lentinan Exerts its Anti-Inflammatory Activity by Suppressing TNFR1 Transfer to the Surface of Intestinal Epithelial Cells through Dectin-1 in an in vitro and mice model.
Immunome Res., 14:165 (2018)

Yamamoto H, Kondo A, Itoh T.
A curvature-dependent membrane binding by tyrosine kinase Fer involves an intrinsically disordered region.
Biochem. Biophys. Res. Commun., 495(1):1522-1527 (2018)

Ando H, Hirose M, Gainche L, Kawaai K, Bonneau B, Ijuin T, Itoh T, Takenawa T, Mikoshiba K.
IRBIT Interacts with the Catalytic Core of Phosphatidylinositol Phosphate Kinase Type Iα and IIα through Conserved Catalytic Aspartate Residues.
PLoS ONE, 10(10):e0141569 (2015)

Tsujita K, Takenawa T, Itoh T.
Feedback regulation between plasma membrane tension and membrane-bending proteins organizes cell polarity during leading edge formation.
Nat. Cell Biol., 17(6), 749-758 (2015)

Tsujita K, Itoh T.
Phosphoinositides in the regulation of actin cortex and cell migration.
Biochim. Biophys. Acta, 1851(6), 824-831 (2015)

Tokuda E, Itoh T, Hasegawa J, Ijuin T, Takeuchi Y, Irino Y, Fukumoto M, Takenawa T.
Phosphatidylinositol 4-phosphate in the Golgi apparatus regulates cell-cell adhesion and invasive cell migration in human breast cancer.
Cancer Res., 74(11), 3054-3066 (2014)

Li C, Kita A, Hashimoto Y, Ihara M, Kato A, Ogura N, Doi A, Oku M, Itoh T, Sakai Y, Sugiura R.
Functional link between Rab GTPase-mediated membrane trafficking and PI4,5P2 signaling.
Genes Cells, 19(3), 177-197 (2014)

Yamazaki D, Itoh T, Miki H, Takenawa T.
srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity.
Mol. Biol. Cell, 24(21), 3393-3405 (2013)

Tsujita K, Kondo A, Kurisu S, Hasegawa J, Itoh T, Takenawa T.
Antagonistic regulation of F-BAR protein assemblies controls actin polymerization during podosome formation.
J. Cell Sci., 126(10), 2267-2278 (2013)

Tanaka-Takiguchi Y, Itoh T, Tsujita K, Yamada S, Yanagisawa M, Fijiwara K, Yamamoto A, Ichikawa M, Takiguchi K.
Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristic of individual F-BAR domain proteins.
Langmuir, 29(1), 328-336 (2013)

Lee HC, Inoue T, Sasaki J, Kubo T, Matsuda S, Nakasaki Y, Hattori M, Tanaka F, Udagawa O, Kono N, Itoh T, Ogiso H, Taguchi R, Arita M, Sasaki T, Arai H.
LPIAT1 regulates arachidonic acid content in phosphatidylinositol and is required for cortical lamination in mice.
Mol. Biol. Cell, 23(24), 4689-4700 (2012)

Itoh T, Hasegawa J.
Mechanistic insights into the regulation of circular dorsal ruffle formation.
J. Biochem. (Tokyo), 153(1), 21-29 (2012)

Hasegawa J, Tsujita K, Takenawa T, Itoh T.
ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf5.
Mol. Biol. Cell, 23(13), 2593-2604 (2012)

Kato K, Yazawa T, Taki K, Mori K, Wang S, Nishioka T, Hamaguchi T, Itoh T, Takenawa T, Kataoka C, Matsuura Y, Amano M, Murohara T, Kaibuchi K.
The inositol 5-phosphatase SHIP2 is an effector of RhoA and is involved in cell polarity and migration.
Mol. Biol. Cell, 23(13), 2481-2489 (2012)

Irino Y, Tokuda E, Hasegawa J, Itoh T, Takenawa T.
Quantification and visualization of phosphoinositides by quantum dot-labeled specific binding-domain probes.
J. Lipid Res., 53(4), 810-819 (2012)

Hasegawa J, Tokuda E, Tenno T, Tsujita K, Sawai H, Hiroaki H, Takenawa T, Itoh T.
SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain.
J. Cell Biol., 193(5), 901-916 (2011)

Uezu A, Umeda K, Tsujita K, Suetsugu S, Takenawa T, Nakanishi H.
Characterization of the EFC/F-BAR domain protein, FCHO2.
Genes Cells, 16(8):868-878 (2011)

Wang S, Ijuin T, Itoh T, Takenawa T.
Regulation of IGF-1/PI3K/Akt signaling by the phosphoinositide phosphatase pharbin.
J. Biochem. (Tokyo), 150(1), 83-93 (2011)

Takano K, Watanabe-Takano H, Suetsugu S, Kurita S, Tsujita K, Kimura S, Karatsu T, Takenawa T, Endo T.
Nebulin and N-WASP cooperate to cause IGF-1-induced sarcomeric actin filament formation.
Science, 330(6010):1536-1540 (2010)

Tsujita K, Itoh T, Kondo A, Oyama M, Kozuka-Hata H, Irino Y, Hasegawa J, Takenawa T.
Proteome of acidic phospholipid-binding proteins: Spatial and temporal regulation of coronin 1A by phosphoinositides.
J. Biol. Chem., 285(9), 6781-6789 (2010)

Takefuji M, Asano H, Mori K, Amano M, Kato K, Watanabe T, Morita Y, Katsumi A, Itoh T, Takenawa T, Hirashiki A, Izawa H, Nagata K, Hirayama H, Takatsu F, Naoe T, Yokota M, Kaibuchi K.
Mutation of ARHGAP9 in patients with coronary spastic angina.
J. Hum. Genet., 55(1), 42-49 (2010)

Yamada H, Padilla-Parra S, Park SJ, Itoh T, Chaineau M, Monaldi I, Cremona O, Benfenati F, De Camilli P, Coppey-Moisan M, Tramier M, Galli T, Takei K.
Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.
J. Biol. Chem., 284(49), 34244-34256 (2009)

Itoh T, Hasegawa J, Tsujita K, Kanaho Y, Takenawa T.
The tyrosine kinase Fer is a downstream target of the PLD-PA pathway that regulates cell migration.
Science Signaling, 2(87), ra52 (2009)

Itoh T, Takenawa T.
Mechanisms of membrane deformation by lipid-binding domains.
Prog. Lipid Res., 48(5), 298-305 (2009) [Review]

Oikawa T, Itoh T, Takenawa T.
Sequential signals toward podosome formation in NIH-src cells.
J. Cell Biol., 182(1), 157-169 (2008)

Uezu A, Horiuchi A, Kanda K, Kikuchi N, Umeda K, Tsujita K, Suetsugu S, Araki N, Yamamoto H, Takenawa T, Nakanishi H.
SGIP1alpha is an endocytic protein that directly interacts with phospholipids and Eps15.
J. Biol. Chem., 282(36):26481-26489 (2007)

Shimada A, Niwa H, Tsujita K, Suetsugu S, Nitta K, Hanawa-Suetsugu K, Akasaka R, Nishino Y, Toyama M, Chen L, Liu ZJ, Wang BC, Yamamoto M, Terada T, Miyazawa A, Tanaka A, Sugano S, Shirouzu M, Nagayama K, Takenawa T, Yokoyama S.
Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis.
Cell, 129(4):761-772 (2007)

Tokuda E, Fujita N, Oh-hara T, Sato S, Kurata A, Katayama R, Itoh T, Takenawa T, Miyazono K, Tsuruo T.
Casein kinase 2-interacting protein-1, a novel Akt pleckstrin homology domain-interacting protein, down-regulates PI3K/Akt signaling and suppresses tumor growth in vivo.
Cancer Res., 67(20), 9666-9676 (2007)

Tsujita K, Suetsugu S, Sasaki N, Furutani M, Oikawa T, Takenawa T.
Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis.
J. Cell Biol., 172(2):269-279 (2006)

Furutani M, Tsujita K, Itoh T, Ijuin T, Takenawa T.
Application of phosphoinositide-binding domains for the detection and quantification of specific phosphoinositides.
Anal. Biochem., 355(1), 8-18 (2006)

Furutani M, Itoh T, Ijuin T, Tsujita K, Takenawa T.
Thin layer chromatography-blotting, a novel method for the detection of phosphoinositides.
J. Biochem. (Tokyo), 139(4), 663-670 (2006)

Itoh T, De Camilli P.
BAR, F-BAR and ENTH/ANTH domains in the regulation of membrane-cytosol interfaces and membrane curvature.
Biochim. Biophys. Acta, 1761(8), 897-912 (2006) [Review]

Takenawa T, Itoh T.
Membrane targeting and remodeling through phosphoinositide-binding domains.
IUBMB Life, 58(5-6), 296-303 (2006) [Review]

Itoh T, Erdmann KS, Roux A, Habermann B, Werner H, De Camilli P.
Dynamin and the Actin Cytoskeleton Cooperatively Regulate Plasma Membrane Invagination by BAR and F-BAR Proteins.
Dev. Cell, 9(6), 791-804 (2005)

Tsujita K, Itoh T, Ijuin T, Yamamoto A, Shisheva A, Laporte J, Takenawa T.
Myotubularin regulates the function of the late endosome through the gram domain-phosphatidylinositol 3,5-bisphosphate interaction.
J. Biol. Chem., 279(14), 13817-13824 (2004)

Oikawa T, Yamaguchi H, Itoh T, Kato M, Ijuin T, Yamazaki D, Suetsugu S, Takenawa T.
PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia.
Nat. Cell Biol., 6(5), 420-426 (2004)

Itoh T, De Camilli P.
Membrane trafficking: dual-key strategy.
Nature, 429(6988), 141-143 (2004) [News & Views]

Itoh T, Takenawa T.
Regulation of endocytosis by phosphatidylinositol 4,5-bisphosphate and ENTH proteins.
Curr. Top. Microbiol. Immunol., 282, 31-47 (2004) [Review]

Otsuki M, Itoh T, Takenawa T.
Neural Wiskott-Aldrich syndrome protein is recruited to rafts and associates with endophilin A in response to epidermal growth factor.
J. Biol. Chem., 278(8), 6461-6469 (2003)

Itoh T, Takenawa T.
Phosphoinositide-binding domains: Functional units for temporal and spatial regulation of intracellular signalling.
Cell Signal., 14(9), 733-743 (2002) [Review]

Itoh T, Koshiba S, Kigawa T, Kikuchi A, Yokoyama S, Takenawa T.
Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis.
Science, 291(5506), 1047-1051 (2001)

Park SJ, Itoh T, Takenawa T.
Phosphatidylinositol 4-phosphate 5-kinase type I is regulated through phosphorylation response by extracellular stimuli.
J. Biol. Chem., 276(7), 4781-4787 (2001)

Takenawa T, Itoh T.
Phosphoinositides, key molecules for regulation of actin cytoskeletal organization and membrane traffic from the plasma membrane.
Biochim. Biophys. Acta, 1533(3), 190-206 (2001) [Review]

Zhang Y, Sugiura R, Lu Y, Asami M, Maeda T, Itoh T, Takenawa T, Shuntoh H, Kuno T.
Phosphatidylinositol 4-phosphate 5-kinase Its3 and calcineurin Ppb1 coordinately regulate cytokinesis in fission yeast.
J. Biol. Chem., 275(45), 35600-35606 (2000)

Itoh T, Ishihara H, Shibasaki Y, Oka Y, Takenawa T.
Autophosphorylation of type I phosphatidylinositol phosphate kinase regulates its lipid kinase activity.
J. Biol. Chem., 275(25), 19389-19394 (2000)

Takenawa T, Itoh T, Fukami K.
Regulation of phosphatidylinositol 4,5-bisphosphate levels and its roles in cytoskeletal re-organization and malignant transformation.
Chem. Phys. Lipids, 98(1-2), 13-22 (1999) [Review]

Yu H, Fukami K, Itoh T, Takenawa T.
Phosphorylation of phospholipase Cgamma1 on tyrosine residue 783 by platelet-derived growth factor regulates reorganization of the cytoskeleton.
Exp. Cell Res., 243(1), 113-122 (1998)

Itoh T, Ijuin T, Takenawa T.
A novel phosphatidylinositol-5-phosphate 4-kinase (phosphatidylinositol-phosphate kinase IIgamma) is phosphorylated in the endoplasmic reticulum in response to mitogenic signals.
J. Biol. Chem., 273(32), 20292-20299 (1998)

Sakisaka T, Itoh T, Miura K, Takenawa T.
Phosphatidylinositol 4,5-bisphosphate phosphatase regulates the rearrangement of actin filaments.
Mol. Cell. Biol., 17(7), 3841-3849 (1997)

Itoh T, Miura K, Miki H, Takenawa T.
Beta-tubulin binds Src homology 2 domains through a region different from the tyrosine-phosphorylated protein-recognizing site.
J. Biol. Chem., 271(44), 27931-27935 (1996)

Toshiki Itoh & Shiro Suetsugu
Chapter 4: "BAR domains"
『Plasma Membrane Shaping』, Academic Press, 2022年, ISBN: 978-0-323-89911-6.

Kazuya Tsujita & Toshiki Itoh
Chapter 19: "Membrane tension and mechanobiology of cell migration"
『Plasma Membrane Shaping』, Academic Press, 2022年, ISBN: 978-0-323-89911-6.

伊藤俊樹
「細胞膜の曲率と張力をめぐる分子機構」
『生体の科学』, 医学書院, 第67巻3号, p214-219, 2016年.

伊藤俊樹 (第7章 分担翻訳)
細胞のシグナル伝達（システムとしての共通原理にもとづく理解）
監訳 西田栄介, メディカルサイエンスインターナショナル, 2016年.

辻田和也、伊藤俊樹
「細胞運動の際の極性の形成における細胞膜の張力と膜変形タンパク質によるフィードバック制御機構」
『ライフサイエンス新着論文レビュー』 (ウェブ出版), 2015年.

伊藤俊樹
「脂質二重層の曲率を認識する分子機構」
『医学のあゆみ・生命を支える脂質~最新の研究と臨床~』, 医歯薬出版株式会社, Vol. 248, No.13, p1069-1074, 2014年.

伊藤俊樹
「エンドサイトーシスにおける細胞膜の形状制御機構」
『生化学』, 第84巻, 第1号, p5-17, 2012年.

伊藤俊樹 (第3、4章 分担翻訳)
リッピンコットシリーズ イラストレイテッド細胞分子生物学
監訳 水島 昇, 丸善出版, 2012年.

竹縄忠臣、伊藤俊樹 (共同編者)
『改訂タンパク質実験ハンドブック』, 羊土社, 2011年.

伊藤俊樹
「脂質二重層の形状を制御する分子機構」
『実験医学増刊・分子から個体へと深化する脂質生物学』 (佐々木雄彦、横溝岳彦、竹縄忠臣 編), 羊土社, p75-78, 2010年.

伊藤俊樹、竹縄忠臣
「生体膜の曲率依存的な細胞内情報伝達機構」
『生体の科学・現代医学 生物学の仮説・学説2008』, 医学書院, p370-371, 2008年.

伊藤俊樹、竹縄忠臣
「スポットアレイ法によるタンパク質－脂質間相互作用の検出」
『実験医学別冊・分子間相互作用解析ハンドブック』 (磯部俊明、中山敬一、伊藤隆司 編), 羊土社, p269-271, 2007年.

伊藤俊樹、竹縄忠臣
「リポソーム共沈法によるタンパク質－脂質間相互作用の検出」
『実験医学別冊・分子間相互作用解析ハンドブック』 (磯部俊明、中山敬一、伊藤隆司 編), 羊土社, p272-274, 2007年.

伊藤俊樹
「生体膜の形状を決定するタンパク～アクチン細胞骨格制御におけるその役割～」
『実験医学増刊・形と運動を司る細胞のダイナミクス』 (竹縄忠臣、遠藤剛 編), 羊土社, p76-85, 2006年.

伊藤俊樹、竹縄忠臣
「細胞内情報伝達」
『脳神経科学』 (監修 伊藤正男、編集 金澤一郎、篠田義一、廣川信隆、御子柴克彦、宮下保司), 三輪書店, p330-345, 2003年.

伊藤俊樹
「イノシトールリン脂質（ホスホイノシチド）によるアクチン細胞骨格系の制御」
『膜（日本膜学会誌）』, Vol.27, No.2, p74-82, 2002年.

伊藤俊樹
「エンドサイトーシスにおけるイノシトールリン脂質の役割」
『生物物理（日本生物物理学会誌）』, 42(3), p131-134, 2002年.

伊藤俊樹
「イノシトールリン脂質」
『細胞骨格と細胞運動 その制御メカニズム』 (竹縄忠臣 編), シュプリンガー・フェアラーク東京, p134-143, 2002年.

伊藤俊樹、竹縄忠臣
「細胞内小胞輸送におけるタンパク質－脂質間相互作用」
『実験医学』, 羊土社, Vol.20, No.16, p2315-2321, 2002年.

伊藤俊樹
「エンドサイトーシスにおけるENTHドメインの役割」
『実験医学』, 羊土社, Vol.19, No.8, p970-972, 2001年.

伊藤俊樹
「脂質結合ドメインを介したイノシトールリン脂質の機能発現―PH・ENTHドメイン、FYVEフィンガー」
『実験医学増刊・プロテオーム研究とシグナル蛋白ドメイン』 (竹縄忠臣 編), 羊土社, Vol.18, No.18, p203-211, 2000年.

伊藤俊樹、竹縄忠臣
「シグナル伝達の時空制御におけるリン脂質の役割」
『実験医学増刊・細胞膜・核内レセプターと脂溶性シグナル分子』 (加藤茂明，清水孝雄 編), 羊土社, Vol.18, No.2, p97-102, 2000年.

伊藤俊樹、竹縄忠臣
「ホスファチジルイノシトールキナーゼ-PI4-キナーゼ、PIPキナーゼの細胞内情報伝達における役割」
『蛋白質核酸酵素・脂質の分子生物学と病態生化学』 (井上圭三・西島正弘 編), 共立出版社, Vol.44, No.8, p949-960, 1999年.

伊藤俊樹、竹縄忠臣
「SH2, SH3, PH各領域を介する蛋白質の会合」
『シグナル伝達実験法』 (宇井理生 編), 羊土社, p205-213, 1996年.

伊藤俊樹
「ウェスタンブロッティング」
『実験医学別冊・新遺伝子工学ハンドブック』 (村松正實、岡山博人、山本雅 編), 羊土社, p222-226, 1996年.

伊藤俊樹
「イノシトールリン脂質によるアクチン細胞骨格の制御」
『実験医学増刊・脂質研究の新展開』 (竹縄忠臣 編), 羊土社, p.136-143, 1996年.

伊藤俊樹
「Far-Western法によるタンパク質-タンパク質間相互作用の検出」
『細胞工学別冊・分子医科学実験プロトコール』 (東京大学医科学研究所学友会 編), 秀潤社, p62-66, 1995年.